Difference between revisions of "Part:BBa C0040:Design"

Revision as of 01:07, 30 April 2006

tetracycline repressor from transposon Tn10 (+LVA)

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

References (unparsed) here:

Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).

Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.   PMID: 9092630

References (unparsed) here:

Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).

Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.   PMID: 9092630

BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.

Difference between revisions of "Part:BBa C0040:Design"

Revision as of 01:07, 30 April 2006

Design Notes

Source

References

Files

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 ===Design Notes===
-References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting      Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.   PMID: 9092630 </p> <P> References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting      Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.   PMID: 9092630 </p> <P>BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator.      It has been modified to include a rapid degradation LVA tail, and includes      the BioBrick standard assembly head and tail restriction sites. The RBS has      been removed. The stop codon has been changed from TAA to a double stop codon      TAATAA.  <P>
+References (unparsed) here:
+Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting      Proteins. Thesis, Princeton Univ., Princeton (1999).
+ Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.   PMID: 9092630
+ References (unparsed) here:
+Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting      Proteins. Thesis, Princeton Univ., Princeton (1999).
+ Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.   PMID: 9092630
+BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator.      It has been modified to include a rapid degradation LVA tail, and includes      the BioBrick standard assembly head and tail restriction sites. The RBS has      been removed. The stop codon has been changed from TAA to a double stop codon      TAATAA.