Difference between revisions of "Part:BBa J176127"
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<partinfo>BBa_J176127 short</partinfo>
 
<partinfo>BBa_J176127 short</partinfo>
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{{Curation/ccdb}}
  
 
V0120, standard high-copy cloning vector for E. coli
 
V0120, standard high-copy cloning vector for E. coli
  
 
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===Usage and Biology===
 
===Usage and Biology===
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This high-copy vector carries RFC23 (Silver Standard) BioBrick cloning sites for modular protein assembly.
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Latest revision as of 17:52, 9 July 2015

V0120

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

V0120, standard high-copy cloning vector for E. coli


Usage and Biology

This high-copy vector carries RFC23 (Silver Standard) BioBrick cloning sites for modular protein assembly.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 101
    Illegal XbaI site found at 116
    Illegal SpeI site found at 1825
    Illegal PstI site found at 1839
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 101
    Illegal SpeI site found at 1825
    Illegal PstI site found at 1839
    Illegal NotI site found at 107
    Illegal NotI site found at 247
    Illegal NotI site found at 1832
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 101
    Illegal BglII site found at 1015
    Illegal BamHI site found at 318
    Illegal BamHI site found at 1021
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 1825
    Illegal EcoRI site found at 101
    Illegal XbaI site found at 116
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 101
    Illegal XbaI site found at 116
    Illegal SpeI site found at 1825
    Illegal PstI site found at 1839
    Illegal NgoMIV site found at 4648
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1571
    Illegal BsaI.rc site found at 3216
    Illegal SapI site found at 2133