Difference between revisions of "Part:BBa K176157"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K176157 short</partinfo>
 
<partinfo>BBa_K176157 short</partinfo>
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{{Curation/ccdb}}
  
 
Add LVA degradation tag (widely used in C0040, C0051, C0061...) to ccdB(K145151) C-terminal:
 
Add LVA degradation tag (widely used in C0040, C0051, C0061...) to ccdB(K145151) C-terminal:

Latest revision as of 17:35, 9 July 2015

ccdB-LVA coding sequence

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

Add LVA degradation tag (widely used in C0040, C0051, C0061...) to ccdB(K145151) C-terminal: 

[K145151 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA]
                              A   A   N   D   E   N   Y   A   L   V   A

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216