Difference between revisions of "Part:BBa K1638009"
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| − | T18 domain of the catalytic active domain of CyaA from Bordetella pertussis linked together with GFP through a flexible 10 aa linker. This indirectly allows detection of correctly transcription and translation. | + | T18 domain of the catalytic active domain of CyaA from Bordetella pertussis linked together with GFP through a flexible 10 aa linker. This indirectly allows detection of correctly transcription and translation of both T18 domain and GFP (by the presence of green fluorescence). |
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Revision as of 14:46, 9 July 2015
T18 domain of cyaA from Bordetella pertussis with GFP reporter
T18 domain of the catalytic active domain of CyaA from Bordetella pertussis linked together with GFP through a flexible 10 aa linker. This indirectly allows detection of correctly transcription and translation of both T18 domain and GFP (by the presence of green fluorescence).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 711
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 252
Illegal NgoMIV site found at 662
Illegal AgeI site found at 468 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1384