Difference between revisions of "Part:BBa K1638004"
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This part codes for the T18 domain of the catalytic domain of the adenylate cyclase, CyaA, from Bordetella pertussis. When associated with the T25 domain of the catalytic domain of CyaA, the two domains together become active and catalyzes the conversion of ATP to cAMP. The rise in cAMP can in turn be used to trigger the expression of certain genes by using a cAMP-induced promoter. | This part codes for the T18 domain of the catalytic domain of the adenylate cyclase, CyaA, from Bordetella pertussis. When associated with the T25 domain of the catalytic domain of CyaA, the two domains together become active and catalyzes the conversion of ATP to cAMP. The rise in cAMP can in turn be used to trigger the expression of certain genes by using a cAMP-induced promoter. | ||
| − | <partinfo>BBa_K1638002</partinfo> | + | |
| + | The T25 domain can be found here: <partinfo>BBa_K1638002</partinfo> | ||
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Revision as of 18:28, 8 July 2015
T18 domain of CyaA from Bordetella pertussis
This part codes for the T18 domain of the catalytic domain of the adenylate cyclase, CyaA, from Bordetella pertussis. When associated with the T25 domain of the catalytic domain of CyaA, the two domains together become active and catalyzes the conversion of ATP to cAMP. The rise in cAMP can in turn be used to trigger the expression of certain genes by using a cAMP-induced promoter.
The T25 domain can be found here: BBa_K1638002
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 550
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 91
Illegal NgoMIV site found at 501
Illegal AgeI site found at 307 - 1000COMPATIBLE WITH RFC[1000]