Difference between revisions of "Part:BBa C0012:Design"
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===Design Notes===
 
===Design Notes===
  
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References (unparsed) here: <p>Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional      regulators. <em>Nature</em> 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a><br>    <br>   </P> <P> References (unparsed) here: <p>Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional      regulators. <em>Nature</em> 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a><br>    <br>   </P> <P>Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained      sequence was compared to the wild-type sequence for LacI obtained through      a database search. The sequence had been modified from the wild-type in that      wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli      has several GTG starts it would seem). The LVA tag has been added for quicker      degradation.<P> Incompatible with systems containing LacI, lactose, or IPTG.
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References (unparsed) here:  
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Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional      regulators. <em>Nature</em> 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a><br>    <br>  
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 +
References (unparsed) here:  
 +
 
 +
Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional      regulators. <em>Nature</em> 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a><br>    <br>  
 +
 
 +
Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained      sequence was compared to the wild-type sequence for LacI obtained through      a database search. The sequence had been modified from the wild-type in that      wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli      has several GTG starts it would seem). The LVA tag has been added for quicker      degradation.
 +
 
 +
Incompatible with systems containing LacI, lactose, or IPTG.
  
  

Revision as of 01:07, 30 April 2006


lacI repressor from E. coli (+LVA)


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

References (unparsed) here:

Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a>

References (unparsed) here:

Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a>

Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. 

Incompatible with systems containing LacI, lactose, or IPTG.


Source

--What is the source of this part?--

References

Files