Difference between revisions of "Part:BBa K1033120"

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===Registry Curation===
 
===Registry Curation===
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Original sequence changed by iGEM HQ, notified by Team [http://2015.igem.org/Team:UMaryland Umaryland 2015 Team]
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Original sequence changed by iGEM HQ
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*'''Change''' - "TAT" added to 5' end.
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*'''Change''' - "TAT" added to start of part sequence, ''notified by Team [http://2015.igem.org/Team:UMaryland UMaryland 2015 Team]''
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*'''Reason''' - To reflect sample sequence
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**'''Reason''' - To reflect sample sequence
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*'''Change''' - Part type from "Coding" to "Translational Unit"
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**'''Reason''' - RBS in front of coding Region
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{{Template:CurationNote|change="TAT" added to start of part sequence|reason=To reflect sample sequence, ''notified by [http://2015.igem.org/Team:UMaryland UMaryland 2015 Team]''}}
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 20:20, 7 July 2015

B0034-ExpTag-Linker-Miraculin-Linker-6xHis

Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum".

This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.

Applications

Miraculin can be used as a sweetener.


Characterization data from Uppsala iGEM 2013

We were not sucessful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.


Registry Curation

Original sequence changed by iGEM HQ

  • Change - "TAT" added to start of part sequence, notified by Team [http://2015.igem.org/Team:UMaryland UMaryland 2015 Team]
    • Reason - To reflect sample sequence
  • Change - Part type from "Coding" to "Translational Unit"
    • Reason - RBS in front of coding Region

HQ Curation

iGEM HQ Staff has curated this part. Curation may add, remove, or alter information on a part, to better represent its use and character. This part may require further curation.

Change: {{{1}}}

  • Reason: {{{2}}}

iGEM users are also encouraged to make their own additions and changes to part pages as contributions. Contact hq (AT) igem (DOT) org with any questions.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]