Difference between revisions of "Part:BBa K1033120:Experience"
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===Applications of BBa_K1033120===
 
===Applications of BBa_K1033120===
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We were sucessful in both subcloning the synthetic construct to a desired plasmid  and isolating the Maraculin gene by itself. The sequencencing was done at GATC biotch and Uppsla Genome center. We were not sucessful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.
 
  
 
===User Reviews===
 
===User Reviews===
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<I>Iowis Zhu</I>
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The UMaryland 2015 team, added a pBAD promoter via 3A assembly. Under arabinose induction, we observed a band of the right monomer weight appear on SDS PAGE. More can be seen in the entry for the composite part: https://parts.igem.org/wiki/index.php/Part:BBa_K1783000
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Latest revision as of 14:47, 1 July 2015

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Please enter how you used this part and how it worked out.

Applications of BBa_K1033120

User Reviews

UNIQ2af03960aef63e9c-partinfo-00000000-QINU UNIQ2af03960aef63e9c-partinfo-00000001-QINU

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Iowis Zhu

The UMaryland 2015 team, added a pBAD promoter via 3A assembly. Under arabinose induction, we observed a band of the right monomer weight appear on SDS PAGE. More can be seen in the entry for the composite part: https://parts.igem.org/wiki/index.php/Part:BBa_K1783000

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