Difference between revisions of "Part:pSB1A7"

m (Part:BBa J31009 moved to Part:pSB1A7)
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_J31009 short</partinfo>
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<partinfo>pSB1A7 short</partinfo>
  
 
pSB1A7 was constructed by adding double terminators ([https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015]) before the BioBrick prefix and after the BioBrick suffix in [https://parts.igem.org/cgi/partsdb/part_info.cgi?part_name=pSB1A3 pSB1A3]. The added terminators block read-through transcription coming from the vector backbone into the BioBrick MCS.
 
pSB1A7 was constructed by adding double terminators ([https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015]) before the BioBrick prefix and after the BioBrick suffix in [https://parts.igem.org/cgi/partsdb/part_info.cgi?part_name=pSB1A3 pSB1A3]. The added terminators block read-through transcription coming from the vector backbone into the BioBrick MCS.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_J31009 SequenceAndFeatures</partinfo>
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<partinfo>pSB1A7 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_J31009 parameters</partinfo>
+
<partinfo>pSB1A7 parameters</partinfo>
 
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Revision as of 15:43, 16 November 2006

Transcriptionally insulated high copy BioBrick plasmid

pSB1A7 was constructed by adding double terminators (BBa_B0015) before the BioBrick prefix and after the BioBrick suffix in pSB1A3. The added terminators block read-through transcription coming from the vector backbone into the BioBrick MCS.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2411
    Illegal XbaI site found at 2426
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2411
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal NotI site found at 8
    Illegal NotI site found at 2417
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2411
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2411
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2426
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1312