Difference between revisions of "Part:BBa K1033120"
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===Registry Curation=== | ===Registry Curation=== | ||
Original sequence changed by iGEM HQ, notified by Team [http://2015.igem.org/Team:UMaryland Umaryland 2015 Team] | Original sequence changed by iGEM HQ, notified by Team [http://2015.igem.org/Team:UMaryland Umaryland 2015 Team] | ||
| − | '''Change''' - "TAT" added to 5' end. | + | *'''Change''' - "TAT" added to 5' end. |
| − | '''Reason''' - To reflect sample sequence | + | *'''Reason''' - To reflect sample sequence |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 14:52, 11 June 2015
B0034-ExpTag-Linker-Miraculin-Linker-6xHis
Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum".
This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.
Applications
Miraculin can be used as a sweetener.
Characterization data from Uppsala iGEM 2013
We were not sucessful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.
Registry Curation
Original sequence changed by iGEM HQ, notified by Team [http://2015.igem.org/Team:UMaryland Umaryland 2015 Team]
- Change - "TAT" added to 5' end.
- Reason - To reflect sample sequence
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]