Difference between revisions of "Part:BBa K1648045:Design"

(→‎Design Notes)
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K1648000 short</partinfo>
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<partinfo>BBa_K1648045 short</partinfo>
  
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<partinfo>BBa_K1648000 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1648045 SequenceAndFeatures</partinfo>
  
  
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===Source===
 
===Source===
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BBa_K1314016
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Synthesis.
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===References===
 
===References===

Revision as of 07:24, 31 May 2015


AvOprF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 475


Design Notes

PCR with BBa_K1314016 as template using the following primers:

>SH3 Domain F
GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCAGAGTATGTGCGGGC

>SH3 Domain R
GTTTCTTCCTGCAGCGGCCGCTACTAGTATACTTCTCCACGTAAGGGACAGG

Source

BBa_K1314016

References