Difference between revisions of "Part:BBa B0030:Design"

 
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===Design Notes===
 
===Design Notes===
  
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<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks      suffix ("orig" in figure 4-14 of Ron Weiss thesis).  <p>No secondary structures are formed in the given RBS region. Users should      check for secondary structures induced in the RBS by upstream and downstream      elements in the +50 to -50 region, as such structures will greatly affect      the strength of the RBS.<P>
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Varies from -6 to +1 region from original sequence to accomodate BioBricks      suffix ("orig" in figure 4-14 of Ron Weiss thesis).   
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No secondary structures are formed in the given RBS region. Users should      check for secondary structures induced in the RBS by upstream and downstream      elements in the +50 to -50 region, as such structures will greatly affect      the strength of the RBS.
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Revision as of 01:07, 30 April 2006


RBS.1 (strong) -- modified from R. Weiss


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis).

No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.



Source

--What is the source of this part?--

References

Files