Difference between revisions of "Part:BBa J119375:Design"

 
(→‎References)
 
(2 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
None.
+
Mutations were introduced into the Ptac promoter using NNNNNN in place of the TTGACA of the -35 region. Synthetic oligonucleotides carry the 4096 possible sequences were cloned via Golden Gate Assembly into [https://parts.igem.org/Part:BBa_J119137 pClone Red].
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
Synthetic oligonucleotides were cloned into pClone Red using BsaI Golden Gate Assembly.
+
Synthetic oligonucleotides cloned with BsaI Golden Gate Assembly into [https://parts.igem.org/Part:BBa_J119137 pClone Red ](BBa_J119137).
  
 
===References===
 
===References===
 +
De Boer, H., Comstock, L., & Vasser, M. (1982). The tac promoter: A functional hybrid derived from the trp and lac promoters. PNAS: 21-25.

Latest revision as of 13:40, 27 March 2015


81 Mutated Ptac Promoters with Varying Strengths


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutations were introduced into the Ptac promoter using NNNNNN in place of the TTGACA of the -35 region. Synthetic oligonucleotides carry the 4096 possible sequences were cloned via Golden Gate Assembly into pClone Red.

Source

Synthetic oligonucleotides cloned with BsaI Golden Gate Assembly into pClone Red (BBa_J119137).

References

De Boer, H., Comstock, L., & Vasser, M. (1982). The tac promoter: A functional hybrid derived from the trp and lac promoters. PNAS: 21-25.