Difference between revisions of "Part:BBa J100204"

Revision as of 20:10, 23 February 2015

actClone Red

We designed actClone Red to test the power of activators. The two BsaI sites allow the reverse primer and transcriptional terminator to be removed and an DNA that binds to an activator to be ligated in their place, causing translation of RFP. This can be used in synthetic biology laboratories or for undergraduate introductory biology lab modules.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1
Illegal PstI site found at 1739
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1
Illegal PstI site found at 1739
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1
Illegal PstI site found at 1739
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1
Illegal PstI site found at 1739
Illegal AgeI site found at 1612
Illegal AgeI site found at 1724
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 928
Illegal BsaI.rc site found at 817
Illegal SapI site found at 708

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_J100204 short</partinfo>
-We designed indClone Red to test the power of inducers. The two BsaI sites allow the reverse primer and transcriptional terminator to be removed and an inducer to be ligated in their place, causing translation of RFP. This can be used in synthetic biology laboratories or for undergraduate introductory biology lab modules.
+We designed actClone Red to test the power of activators. The two BsaI sites allow the reverse primer and transcriptional terminator to be removed and an DNA that binds to an activator to be ligated in their place, causing translation of RFP. This can be used in synthetic biology laboratories or for undergraduate introductory biology lab modules.
 <!-- Add more about the biology of this part here