Difference between revisions of "Part:BBa M36939:Experience"
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Organism: E. coli, | Organism: E. coli, | ||
Device Type: LCPUFA Actuator. | Device Type: LCPUFA Actuator. | ||
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Barcode#: 133024994, | Barcode#: 133024994, | ||
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Organism: E. coli, | Organism: E. coli, | ||
Device Type: LCPUFA Actuator. | Device Type: LCPUFA Actuator. | ||
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| + | '''Performance Data:''' | ||
| + | |||
| + | '''Protein Presence Test: GFP Fluorescence Reading''' | ||
| + | |||
| + | Note: GFP Fluorescence Readings were not taken for this composite part but for its sister part BBa_M36938 (contains Comet GFP). We suspect that similar results would be obtained for this construct (excluding Comet GFP). This data is included simply to provide a full analysis of the mechanism. | ||
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| + | [[File:GFPchart.gif]] | ||
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| + | [[File:GFPgraph.gif]] | ||
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| + | Conclusion: The target protein (enoyl-[acyl-carrier-protein] reductase) is synthesized within the E. coli cells. This result is statistically significant. | ||
| + | |||
| + | |||
| + | '''Protein Functionality Test: pH Reading''' | ||
| + | |||
| + | Functional (enoyl-[acyl-carrier-protein] reductase) is expected to partake in a naturally occurring reaction within the E. coli cell. This reaction consumes hydrogen ions. Thus a functional protein is expected to increase the overall pH of the cell. | ||
| + | |||
| + | ---- | ||
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| + | [[File:pHchart.jpg]] | ||
| + | |||
| + | [[File:phgraph.jpg]] | ||
| + | |||
| + | Conclusion: Production of the target protein (enoyl-[acyl-carrier-protein] reductase) causes an increase in pH, as is to be expected. Therefore we conclude that the target protein is functional. This is statistically significant. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 07:12, 4 December 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_M36939
Stanford Location:
Box Name: BIOE44 S14
Glycerol Freezer Stocks:
Barcode#: 133010734, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Barcode#: 133009653, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Barcode#: 133026955, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Barcode#: 133024660, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Barcode#: 133024658, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Barcode#: 133024682, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Barcode#: 133024673, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Barcode#: 133024994, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Performance Data:
Protein Presence Test: GFP Fluorescence Reading
Note: GFP Fluorescence Readings were not taken for this composite part but for its sister part BBa_M36938 (contains Comet GFP). We suspect that similar results would be obtained for this construct (excluding Comet GFP). This data is included simply to provide a full analysis of the mechanism.
Conclusion: The target protein (enoyl-[acyl-carrier-protein] reductase) is synthesized within the E. coli cells. This result is statistically significant.
Protein Functionality Test: pH Reading
Functional (enoyl-[acyl-carrier-protein] reductase) is expected to partake in a naturally occurring reaction within the E. coli cell. This reaction consumes hydrogen ions. Thus a functional protein is expected to increase the overall pH of the cell.
Conclusion: Production of the target protein (enoyl-[acyl-carrier-protein] reductase) causes an increase in pH, as is to be expected. Therefore we conclude that the target protein is functional. This is statistically significant.
User Reviews
UNIQ8f521d426ebcb140-partinfo-00000000-QINU UNIQ8f521d426ebcb140-partinfo-00000001-QINU