Difference between revisions of "Part:BBa K1432001:Experience"
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===Applications of BBa_K1432001=== | ===Applications of BBa_K1432001=== | ||
| + | We will construct a mlr promoter-GPF-mlrA cluster and clone it into the E. coli-L. lactis shuttle expression vector. So it can be easily constructed in the laboratory and could avoid secondary pollution in the natural environment of E.coli. | ||
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| + | we synthesized mlrA gene, there are 336 codons in Sphingomonas species ACM-3962. We replace 246 of them in order to increase the expression of mlrA because they are not abundant codons in Lactococcus lactis. | ||
| + | And we successfully did it both in E.coli and L.lactis. When we induced the expression of protein, it shows that L.lactis could be more efficiently expressed than E.coli. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 21:55, 2 November 2014
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Applications of BBa_K1432001
We will construct a mlr promoter-GPF-mlrA cluster and clone it into the E. coli-L. lactis shuttle expression vector. So it can be easily constructed in the laboratory and could avoid secondary pollution in the natural environment of E.coli.
we synthesized mlrA gene, there are 336 codons in Sphingomonas species ACM-3962. We replace 246 of them in order to increase the expression of mlrA because they are not abundant codons in Lactococcus lactis. And we successfully did it both in E.coli and L.lactis. When we induced the expression of protein, it shows that L.lactis could be more efficiently expressed than E.coli.
User Reviews
UNIQ71ae4fd192ec7c50-partinfo-00000000-QINU UNIQ71ae4fd192ec7c50-partinfo-00000001-QINU