Difference between revisions of "Part:BBa K1471009:Design"

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===Design Notes===
 
===Design Notes===
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sdgsdfg
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This part was designed to produce the complete protein complex for PhyB comformations and the red lgiht switch system.
  
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===Source===
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Arabidopsis thaliana genome
  
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===References===
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Taiz,L. and E.Zeiger.(2006) Fisiologia Vegetal.Universitat Jaume. 3rd Edition.Pages 7-27
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Rangan, et al. (2008). Analysis of Context Sequence Surrounding Translation Initiation Site from Complete Genome of
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Model Plants. New York University. [Online] Retrieved october 14th 2014 from: http://www.nyu.edu/projects/vogel/Reprints/Rangan_MolBt08.pdf Nakagawa, et al. (2007). Diversity of preferred nucleotide sequences around the translation initiation codon in eukaryote genomes. Oxford University Press. [Online] Retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2241899/ Liu Q, Xue Q. (2005).
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Comparative studies on sequence characteristics around translation initiation codon in four eukaryotes. Zhejiang University. [Online] Retrieved october 14th 2014 from: http://www.ias.ac.in/jgenet/Vol84No3/317.pdf Kozak, M. (1989). Circumstances and mechanisms of inhibition of translation by secondary structure in eucaryotic mRNAs.
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American Society for Microbiology (ASM). [Online] retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC363665/
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Classical Nuclear Localization Signals: Definition, Function, and Interaction with Importin α*. (n.d.). Classical Nuclear Localization Signals: Definition, Function, and Interaction with Importin α. Retrieved October 11, 2014, from http://www.jbc.org/content/282/8/5101.full
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Nuclear localization sequence. (2014, November 10). Wikipedia. Retrieved October 11, 2014, from http://en.wikipedia.org/wiki/Nuclear_localization_sequence Result Filters. (n.d.).
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National Center for Biotechnology Information. Retrieved October 11, 2014, from http://www.ncbi.nlm.nih.gov/pubmed/8241603 Poly(A)-tail profiling reveals an embryonic switch in translational
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control. (n.d.). Nature journal. Retrieved October 11, 2014, from http://www.nature.com/nature/journal/v508/n7494/full/nature13007.html
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The Poly(A) Tail. (n.d.). The RNA Underworld. Retrieved October 12, 2014, from http://aghunt.wordpress.com/2008/06/05/the-polya-tail/ poly-A tail. (n.d.). Nature.com. Retrieved October 12, 2014, from http://www.nature.com/scitable/definition/poly-a-tail-276
  
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===Source===
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Tiley LS1, Madore SJ, Malim MH, Cullen BR.(1992).The VP16 Transcription Activation Domain Is Functional When Targeted to a Promoter-proximal RNA Sequence. Genes Development. Volume 6; Issue 11; Pages: 2077-2087.
  
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sdgsdgfssd
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Carbone, A-L, M. Moroni, P-J Groot-Kormelink, and I. Bermudez(2009).Pentameric concatenated (α4)2(β2)3 and (α4)3(β2)2 nicotinic acetylcholine receptors: subunit arrangement determines functional expression. Britain Journal of Pharmacology.Volue 156; Issue 6; Pages 970–981.
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===References===
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Latest revision as of 21:43, 2 November 2014

RBS - PhyB - AGS - VP16 - NLS - PolyA.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 507
    Illegal BamHI site found at 589
    Illegal XhoI site found at 540
    Illegal XhoI site found at 559
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 756


Design Notes

This part was designed to produce the complete protein complex for PhyB comformations and the red lgiht switch system.

Source

Arabidopsis thaliana genome

References

Taiz,L. and E.Zeiger.(2006) Fisiologia Vegetal.Universitat Jaume. 3rd Edition.Pages 7-27 Rangan, et al. (2008). Analysis of Context Sequence Surrounding Translation Initiation Site from Complete Genome of Model Plants. New York University. [Online] Retrieved october 14th 2014 from: http://www.nyu.edu/projects/vogel/Reprints/Rangan_MolBt08.pdf Nakagawa, et al. (2007). Diversity of preferred nucleotide sequences around the translation initiation codon in eukaryote genomes. Oxford University Press. [Online] Retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2241899/ Liu Q, Xue Q. (2005). Comparative studies on sequence characteristics around translation initiation codon in four eukaryotes. Zhejiang University. [Online] Retrieved october 14th 2014 from: http://www.ias.ac.in/jgenet/Vol84No3/317.pdf Kozak, M. (1989). Circumstances and mechanisms of inhibition of translation by secondary structure in eucaryotic mRNAs. American Society for Microbiology (ASM). [Online] retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC363665/ Classical Nuclear Localization Signals: Definition, Function, and Interaction with Importin α*. (n.d.). Classical Nuclear Localization Signals: Definition, Function, and Interaction with Importin α. Retrieved October 11, 2014, from http://www.jbc.org/content/282/8/5101.full Nuclear localization sequence. (2014, November 10). Wikipedia. Retrieved October 11, 2014, from http://en.wikipedia.org/wiki/Nuclear_localization_sequence Result Filters. (n.d.). National Center for Biotechnology Information. Retrieved October 11, 2014, from http://www.ncbi.nlm.nih.gov/pubmed/8241603 Poly(A)-tail profiling reveals an embryonic switch in translational control. (n.d.). Nature journal. Retrieved October 11, 2014, from http://www.nature.com/nature/journal/v508/n7494/full/nature13007.html The Poly(A) Tail. (n.d.). The RNA Underworld. Retrieved October 12, 2014, from http://aghunt.wordpress.com/2008/06/05/the-polya-tail/ poly-A tail. (n.d.). Nature.com. Retrieved October 12, 2014, from http://www.nature.com/scitable/definition/poly-a-tail-276

Tiley LS1, Madore SJ, Malim MH, Cullen BR.(1992).The VP16 Transcription Activation Domain Is Functional When Targeted to a Promoter-proximal RNA Sequence. Genes Development. Volume 6; Issue 11; Pages: 2077-2087.

Carbone, A-L, M. Moroni, P-J Groot-Kormelink, and I. Bermudez(2009).Pentameric concatenated (α4)2(β2)3 and (α4)3(β2)2 nicotinic acetylcholine receptors: subunit arrangement determines functional expression. Britain Journal of Pharmacology.Volue 156; Issue 6; Pages 970–981.