Difference between revisions of "Part:BBa K1398004"

Latest revision as of 21:13, 2 November 2014

NemR Intergenic Reporter

A construct created to test the effectiveness of the NemR upstream intergenic region (BBa_1398005). The construct contains the entire NemR upstream region (BBa_1398005), which contains a promoter and RBS. It is followed by the fluorescent reporter iLOV (listed in the repository as BBa_K660004), a double STOP codon and a double terminator (B0015).

No fluorescence response was observed when TNT was introduced in solution with the plasmid carrying bacteria (NemR Promoter in graph). The background fluorescence activity of the NemR promoter was higher than the wild type control cells.

Further information on the synthetic promoter can be found here:

https://parts.igem.org/Part:BBa_K1398007

The full article detailing the characterisation of this part can be found here:

http://2014.igem.org/Team:Exeter/Detection

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 The construct contains the entire NemR upstream region (BBa_1398005), which contains a promoter and RBS. It is followed by the fluorescent reporter iLOV (listed in the repository as BBa_K660004), a double STOP codon and a double terminator (B0015).
-No fluorescence response was observed when TNT was introduced in solution with the plasmid carrying bacteria (NemR Promoter in graph). Though the background fluorescence activity of the NemR promoter was higher than the wild type control cells.
+No fluorescence response was observed when TNT was introduced in solution with the plasmid carrying bacteria (NemR Promoter in graph). The background fluorescence activity of the NemR promoter was higher than the wild type control cells.
 https://static.igem.org/mediawiki/parts/9/91/Exeter2014_007_InducableTNTpromoter.jpg