Difference between revisions of "Part:BBa K1470007"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | <p>The 2013 iGEM Freiburg 2013 biobricked an inactive Cas9 (dCas9), which binds to DNA but cannot cleave it anymore and fuctions as a DNA binding protein [ | + | <p>The 2013 iGEM Freiburg 2013 biobricked an inactive Cas9 (dCas9), which binds to DNA but cannot cleave it anymore and fuctions as a DNA binding protein [1]. We improved this enzyme by a single nucleotide mutation to a nickase. The Cas9-Nickase is capable to induce single strand breaks in the genome, thus leading to a simple method of gene editing 2]. </p> |
<p> | <p> | ||
| − | [ | + | [1] https://parts.igem.org/Part:BBa_K1150000<br> [2] Cong, L., Ran, F.A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P.D., Wu, X., Jiang, W., Marraffini, L.A., Zhang, F. (2013). Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 339 (6121), 819-23<br> |
</p> | </p> | ||
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Latest revision as of 21:02, 2 November 2014
Cas9-Nickase
Cas9-Nickase
Usage and Biology
The 2013 iGEM Freiburg 2013 biobricked an inactive Cas9 (dCas9), which binds to DNA but cannot cleave it anymore and fuctions as a DNA binding protein [1]. We improved this enzyme by a single nucleotide mutation to a nickase. The Cas9-Nickase is capable to induce single strand breaks in the genome, thus leading to a simple method of gene editing 2].
[1] https://parts.igem.org/Part:BBa_K1150000
[2] Cong, L., Ran, F.A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P.D., Wu, X., Jiang, W., Marraffini, L.A., Zhang, F. (2013). Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 339 (6121), 819-23
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 248
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]