Difference between revisions of "Part:BBa K1470007"

Latest revision as of 21:02, 2 November 2014

Cas9-Nickase

Usage and Biology

The 2013 iGEM Freiburg 2013 biobricked an inactive Cas9 (dCas9), which binds to DNA but cannot cleave it anymore and fuctions as a DNA binding protein [1]. We improved this enzyme by a single nucleotide mutation to a nickase. The Cas9-Nickase is capable to induce single strand breaks in the genome, thus leading to a simple method of gene editing 2].

[1] https://parts.igem.org/Part:BBa_K1150000
[2] Cong, L., Ran, F.A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P.D., Wu, X., Jiang, W., Marraffini, L.A., Zhang, F. (2013). Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 339 (6121), 819-23

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 248
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1470007 short</partinfo>
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 Cas9-Nickase
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+<p>The 2013 iGEM Freiburg 2013 biobricked an inactive Cas9 (dCas9), which binds to DNA but cannot cleave it anymore and fuctions as a DNA binding protein [1]. We improved this enzyme by a single nucleotide mutation to a nickase. The Cas9-Nickase is capable to induce single strand breaks in the genome, thus leading to a simple method of gene editing 2]. </p>
+<p>
+[1] https://parts.igem.org/Part:BBa_K1150000<br> [2] Cong, L., Ran, F.A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P.D., Wu, X., Jiang, W., Marraffini, L.A., Zhang, F. (2013). Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 339 (6121), 819-23<br>
+</p>
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>