Difference between revisions of "Part:BBa K1398007"
 
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A construct created to test the effectiveness of the NemR recognizing promoter (BBa_1398008).
 
A construct created to test the effectiveness of the NemR recognizing promoter (BBa_1398008).
 
The construct begins with the synthetic promoter NemR, which combines a high-expression promoter with the NemR recognition box (BBa_K1398008). It is followed by a strong RBS (BBa_B0034), the fluorescent reporter iLOV (BBa_K660004), a double STOP codon and a double terminator made up of BBa_B0010 and BBa_B0012.
 
The construct begins with the synthetic promoter NemR, which combines a high-expression promoter with the NemR recognition box (BBa_K1398008). It is followed by a strong RBS (BBa_B0034), the fluorescent reporter iLOV (BBa_K660004), a double STOP codon and a double terminator made up of BBa_B0010 and BBa_B0012.
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Fluorescence response per OD is displayed below:
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https://static.igem.org/mediawiki/parts/9/91/Exeter2014_007_InducableTNTpromoter.jpg
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The synthetic promoter has a higher background fluorescence protein translation compared to the control cells. The expression level of the synthetic promoter rises to the expression level of the upstream intergenic NemR promoter with exposure to trinitrotoluene.
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 +
The full article can be found here:
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http://2014.igem.org/Team:Exeter/Detection
  
 
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<partinfo>BBa_K1398007 parameters</partinfo>
 
<partinfo>BBa_K1398007 parameters</partinfo>
 
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https://static.igem.org/mediawiki/parts/9/91/Exeter2014_007_InducableTNTpromoter.jpg
 

Latest revision as of 20:59, 2 November 2014

NemR Promoter Reporter

A construct created to test the effectiveness of the NemR recognizing promoter (BBa_1398008). The construct begins with the synthetic promoter NemR, which combines a high-expression promoter with the NemR recognition box (BBa_K1398008). It is followed by a strong RBS (BBa_B0034), the fluorescent reporter iLOV (BBa_K660004), a double STOP codon and a double terminator made up of BBa_B0010 and BBa_B0012.

Fluorescence response per OD is displayed below:

Exeter2014_007_InducableTNTpromoter.jpg

The synthetic promoter has a higher background fluorescence protein translation compared to the control cells. The expression level of the synthetic promoter rises to the expression level of the upstream intergenic NemR promoter with exposure to trinitrotoluene.

The full article can be found here:

http://2014.igem.org/Team:Exeter/Detection

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]