Difference between revisions of "Part:BBa K1398004"
EdwardMuir (Talk | contribs) |
EdwardMuir (Talk | contribs) |
||
Line 5: | Line 5: | ||
The construct contains the entire NemR upstream region (BBa_1398005), which contains a promoter and RBS. It is followed by the fluorescent reporter iLOV (listed in the repository as BBa_K660004), a double STOP codon and a double terminator (B0015). | The construct contains the entire NemR upstream region (BBa_1398005), which contains a promoter and RBS. It is followed by the fluorescent reporter iLOV (listed in the repository as BBa_K660004), a double STOP codon and a double terminator (B0015). | ||
− | No fluorescence response was observed when TNT was introduced in solution with the plasmid carrying bacteria. | + | No fluorescence response was observed when TNT was introduced in solution with the plasmid carrying bacteria (004). |
https://static.igem.org/mediawiki/parts/1/11/Flu-OF_compared_to_0ul_TNT_as_the_level_of_TNT_in_a_culture_changes..png | https://static.igem.org/mediawiki/parts/1/11/Flu-OF_compared_to_0ul_TNT_as_the_level_of_TNT_in_a_culture_changes..png | ||
+ | |||
+ | Further information of part 007 can be found here: | ||
+ | |||
+ | https://parts.igem.org/Part:BBa_K1398007 | ||
The full article detailing the characterisation of this part can be found here: | The full article detailing the characterisation of this part can be found here: |
Revision as of 19:52, 2 November 2014
NemR Intergenic Reporter
A construct created to test the effectiveness of the NemR upstream intergenic region (BBa_1398005). The construct contains the entire NemR upstream region (BBa_1398005), which contains a promoter and RBS. It is followed by the fluorescent reporter iLOV (listed in the repository as BBa_K660004), a double STOP codon and a double terminator (B0015).
No fluorescence response was observed when TNT was introduced in solution with the plasmid carrying bacteria (004).
Further information of part 007 can be found here:
https://parts.igem.org/Part:BBa_K1398007
The full article detailing the characterisation of this part can be found here:
http://2014.igem.org/Team:Exeter/Detection
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]