Difference between revisions of "Part:BBa K1398008"
 
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This sequences combines a high level constitutive promoter with the NemR binding box, which allows NemR to bind to DNA when no TNT is present in the cell. It was created for use as a TNT-detection mechanism.
 
This sequences combines a high level constitutive promoter with the NemR binding box, which allows NemR to bind to DNA when no TNT is present in the cell. It was created for use as a TNT-detection mechanism.
 
It combines BBa_J23100 with the NemR box to create a promoter that will theoretically have high levels of transcription when TNT is not present.
 
It combines BBa_J23100 with the NemR box to create a promoter that will theoretically have high levels of transcription when TNT is not present.
 +
 +
An iLOV reporter gene was added upstream of the promoter. The resulting construct is characterised here:
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 +
https://parts.igem.org/Part:BBa_K1398007
 +
 +
The full article can be found here:
 +
 +
http://2014.igem.org/Team:Exeter/Detection
  
  

Latest revision as of 19:06, 2 November 2014

NemR Recognition Promoter

This sequences combines a high level constitutive promoter with the NemR binding box, which allows NemR to bind to DNA when no TNT is present in the cell. It was created for use as a TNT-detection mechanism. It combines BBa_J23100 with the NemR box to create a promoter that will theoretically have high levels of transcription when TNT is not present.

An iLOV reporter gene was added upstream of the promoter. The resulting construct is characterised here:

https://parts.igem.org/Part:BBa_K1398007

The full article can be found here:

http://2014.igem.org/Team:Exeter/Detection


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]