Difference between revisions of "Part:BBa K1398008"

Latest revision as of 19:06, 2 November 2014

NemR Recognition Promoter

This sequences combines a high level constitutive promoter with the NemR binding box, which allows NemR to bind to DNA when no TNT is present in the cell. It was created for use as a TNT-detection mechanism. It combines BBa_J23100 with the NemR box to create a promoter that will theoretically have high levels of transcription when TNT is not present.

An iLOV reporter gene was added upstream of the promoter. The resulting construct is characterised here:

https://parts.igem.org/Part:BBa_K1398007

The full article can be found here:

http://2014.igem.org/Team:Exeter/Detection

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1398008 short</partinfo>
 This sequences combines a high level constitutive promoter with the NemR binding box, which allows NemR to bind to DNA when no TNT is present in the cell. It was created for use as a TNT-detection mechanism.
-It combines BBa_J231000 with the NemR box to create a promoter that will theoretically have high levels of transcription when TNT is not present.
+It combines BBa_J23100 with the NemR box to create a promoter that will theoretically have high levels of transcription when TNT is not present.
+An iLOV reporter gene was added upstream of the promoter. The resulting construct is characterised here:
+https://parts.igem.org/Part:BBa_K1398007
+The full article can be found here:
+http://2014.igem.org/Team:Exeter/Detection