Difference between revisions of "Part:BBa K1398007"

Revision as of 18:15, 2 November 2014

NemR Promoter Reporter

A construct created to test the effectiveness of the NemR recognizing promoter (BBa_1398008). The construct begins with the synthetic promoter NemR, which combines a high-expression promoter with the NemR recognition box (BBa_K1398008). It is followed by a strong RBS (BBa_B0034), the fluorescent reporter iLOV (BBa_K660004), a double STOP codon and a double terminator made up of BBa_B0010 and BBa_B0012.

Fluorescence response per OD is displayed below:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 A construct created to test the effectiveness of the NemR recognizing promoter (BBa_1398008).
 The construct begins with the synthetic promoter NemR, which combines a high-expression promoter with the NemR recognition box (BBa_K1398008). It is followed by a strong RBS (BBa_B0034), the fluorescent reporter iLOV (BBa_K660004), a double STOP codon and a double terminator made up of BBa_B0010 and BBa_B0012.
+Fluorescence response per OD is displayed below:
+https://static.igem.org/mediawiki/parts/9/91/Exeter2014_007_InducableTNTpromoter.jpg
 <!-- Add more about the biology of this part here
@@ Line 17: / Line 21: @@
 <partinfo>BBa_K1398007 parameters</partinfo>
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-https://static.igem.org/mediawiki/parts/9/91/Exeter2014_007_InducableTNTpromoter.jpg