Difference between revisions of "Part:BBa K1321318"

Revision as of 15:23, 2 November 2014

Anderson J23114-RFP in pSEVA331-Bb

This is Anderson promoter J23114 driving the expression of Elowitz RBS-mFRP in pSEVA331-Bb plasmid backbone (part BBa_K1321300) and is a member of the G.xylinus genetic engineering toolkit (parts BBa_K1321295 - BBa_K1321332). RFP expression from BBa_K1321318 has been confirmed in E.coli. BBa_K1321332 is currently being characterized in G.xylinus.

G.xylinus toolkit was designed to ease genetic engineering of cellulose-based biomaterials using the cellulose synthesizing bacterium Gluconacetobacter xylinus (parts Bba_K1321305 and BBa_K1321306). As no registry parts had been tested in G.xylinus, the aim of this toolkit was to determine the parts usable in G.xylinus and to characterize them in this host. pSEVA331-Bb is a non-standard broad host range plasmid capable of replication in G.xylinus and E.coli (a shuttle vector) and was selected because the registry's standard plasmid backbone pSB1C3 can not be used for G.xylinus engineering.

NOTE: Because the registry's standard plasmid backbone pSB1C3 is not capable of replication in Gluconacetobacter species, the G.xylinus genetic engineering toolkit is housed mainly in pSEVA331-Bb. pSEVA331-Bb is a non-standard backbone, which therefore can't be quality controlled by and maintained in the Registry. However, in order to make the G.xylinus toolkit available for the synthetic biology community, Imperial iGEM 2014 team has made it freely available upon request, with quality control provided (see Experience). To request, please contact Imperial iGEM 2014 team.

Sequence and Features