Difference between revisions of "Part:BBa K1321360"

Latest revision as of 06:06, 2 November 2014

CBDclos fused to sfGFP driven by LacI

A LacI-promoter expression construct of super-folder GFP fused C-terminally to CBDclos (a cellulose-binding domain).

This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below:

Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.

For reference, the cellulose binding domain binding capability of CBDclos (N-terminally fused) to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen here (here) - K1321346.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 230
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 551

@@ Line 3: / Line 3: @@
 A LacI-promoter expression construct of super-folder GFP fused C-terminally to CBDclos (a cellulose-binding domain).
+[[File:IC-2014_glow1.jpg |200px|left|]]
 This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below: [[File:IC14-sfGFP-part-table.PNG]]
@@ Line 8: / Line 12: @@
 Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.
+For reference, the cellulose binding domain binding capability of CBDclos (N-terminally fused) to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen here ([https://parts.igem.org/Part:BBa_K1321346 here])  - K1321346.