Difference between revisions of "Part:BBa K1321154"

 
 
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<partinfo>BBa_K1321154 short</partinfo>
 
<partinfo>BBa_K1321154 short</partinfo>
  
GATC
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Metallothionein fMT (metal-binding peptide) fused C-terminally to CBDclos (a cellulose-binding domain).
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This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-fMT-part-table.PNG]]
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Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
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For reference, the cellulose binding domain binding capability of CBDclos (N-terminally fused) to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen ([https://parts.igem.org/Part:BBa_K1321346 here])  - K1321346.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 06:04, 2 November 2014

CBDclos fused to metallothionein Fmt

Metallothionein fMT (metal-binding peptide) fused C-terminally to CBDclos (a cellulose-binding domain).

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: IC14-fMT-part-table.PNG

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

For reference, the cellulose binding domain binding capability of CBDclos (N-terminally fused) to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen (here) - K1321346.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]