Difference between revisions of "Part:BBa K1321093"

Latest revision as of 05:10, 2 November 2014

Phytochelatin (PC) EC20 + Linker-CBDcipA-Linker

Synthetic phytochelatin EC20 (metal binding peptide) fused N-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N-terminal and C-terminal linker sequence.

At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below:

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

For reference the cellulose binding domain binding capability to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen here - K1321371.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 277
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 277
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 277
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 277
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 277
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1321093 short</partinfo>
-GATC
+Synthetic phytochelatin EC20 (metal binding peptide) fused N-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N-terminal and C-terminal linker sequence.
+At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.
+This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-PC-part-table.PNG]]
+Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
+For reference the cellulose binding domain binding capability to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen [https://parts.igem.org/Part:BBa_K1321371 here]  - K1321371.
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