Difference between revisions of "Part:BBa K1332010"
| Line 8: | Line 8: | ||
<b>Figure 1. mRNA circularization device (3'side)(not endless translation)</b><br><br> | <b>Figure 1. mRNA circularization device (3'side)(not endless translation)</b><br><br> | ||
Part which surrounded by red closing line is <b>mRNA circularization device (3’side)(not endless translation)</b>.(figure 1)<br> | Part which surrounded by red closing line is <b>mRNA circularization device (3’side)(not endless translation)</b>.(figure 1)<br> | ||
| − | + | This part has a stop codon, so a circular mRNA made by this part <b>doesn’t cause continuous translation</b>. | |
<h3>How to use</h3> | <h3>How to use</h3> | ||
Revision as of 04:56, 2 November 2014
mRNA circularization device (3 side)
This part consists of the 5' side of the intron(+exon fragment) from td gene of T4 phage(BBa_K1332004) and a double terminator(BBa_B0015). The protein coding sequence that is inserted between this device and mRNA circularization device (5’ side)(BBa_K1332008) can be circularized. The 5' side of the intron(+exon fragment) from td gene of T4 phage contains a stop codon, so a circular mRNA made by this part doesn’t cause continuous translation.
explanation of this part
Figure 1. mRNA circularization device (3'side)(not endless translation)
Part which surrounded by red closing line is mRNA circularization device (3’side)(not endless translation).(figure 1)
This part has a stop codon, so a circular mRNA made by this part doesn’t cause continuous translation.
How to use
Insert the protein coding sequence between this device and mRNA circularization device (5’ side). How to use this part is same as how to use 5' side device(BBa_K1332008) or 3' side device(endless translation)(BBa_K1332009) . Thus,read that.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]