Difference between revisions of "Part:BBa K1363003"
 
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<p>This part is a blue sensor in our project's blue system.We mount the Origin of transfer for the R-plasmid nic region to the part BBa_K592016 to make it can be transfered into the Caulobacter crescentus.So we can let the Caulobacter crescentus sensor the blue light.</p>
 
<h3>Results</h3>
 
<h3>Results</h3>
 
<p>It’s used in our conjugation experiment:</p>
 
<p>It’s used in our conjugation experiment:</p>

Latest revision as of 04:06, 2 November 2014

OriT-K592016 the blue sensor which can be transfered into other bacterias by S17-1

This part is a blue sensor in our project's blue system.We mount the Origin of transfer for the R-plasmid nic region to the part BBa_K592016 to make it can be transfered into the Caulobacter crescentus.So we can let the Caulobacter crescentus sensor the blue light.

Results

It’s used in our conjugation experiment:

The amiICP proteins have an over-expression in C.Crescentus.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 117
    Illegal NgoMIV site found at 127
    Illegal NgoMIV site found at 941
    Illegal NgoMIV site found at 1013
    Illegal NgoMIV site found at 1103
    Illegal NgoMIV site found at 1121
    Illegal NgoMIV site found at 1633
    Illegal AgeI site found at 655
    Illegal AgeI site found at 1801
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1690
    Illegal BsaI.rc site found at 554
    Illegal SapI site found at 19