Difference between revisions of "Part:BBa K1314001"

m
 
Line 2: Line 2:
 
<partinfo>BBa_K1314001 short</partinfo>
 
<partinfo>BBa_K1314001 short</partinfo>
  
−
nifH promoter with RFP reporter
+
nifH promoter with RFP reporter.
  
  
 
===Usage and Biology===
 
===Usage and Biology===
 +
Bba_K1314001 is comprised of nifH promoter and Bba_E1010. It is designed for testing nifH promoter. The right tube shows the expression of RFP using ptep promoter in Dh5a and the left tube show the expression of BbaK1314001 in DH5a. The result show that nifH promoter is not working in DH5a.
 +
[[File:NifH E1010.jpg|200px]]
 +
 +
====Characterization of Bba_K1314001, Bba_K1314013, Bba_K1314014 in Azotobacter vinelandii strain DJ====
 
[[File:Picture2.png|800px]]
 
[[File:Picture2.png|800px]]
−
 
−
 
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'></span>
 
<partinfo>BBa_K1314001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1314001 SequenceAndFeatures</partinfo>
  

Latest revision as of 04:04, 2 November 2014

nifH promoter with mRFP reporter

nifH promoter with RFP reporter.


Usage and Biology

Bba_K1314001 is comprised of nifH promoter and Bba_E1010. It is designed for testing nifH promoter. The right tube shows the expression of RFP using ptep promoter in Dh5a and the left tube show the expression of BbaK1314001 in DH5a. The result show that nifH promoter is not working in DH5a. NifH E1010.jpg

Characterization of Bba_K1314001, Bba_K1314013, Bba_K1314014 in Azotobacter vinelandii strain DJ

Picture2.png


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 801
    Illegal AgeI site found at 913
  • 1000
    COMPATIBLE WITH RFC[1000]