Difference between revisions of "Part:BBa K1314001"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1314001 short</partinfo> | <partinfo>BBa_K1314001 short</partinfo> | ||
| − | nifH promoter with RFP reporter | + | nifH promoter with RFP reporter. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | Bba_K1314001 is comprised of nifH promoter and Bba_E1010. It is designed for testing nifH promoter. The right tube shows the expression of RFP using ptep promoter in Dh5a and the left tube show the expression of BbaK1314001 in DH5a. The result show that nifH promoter is not working in DH5a. | ||
| + | [[File:NifH E1010.jpg|200px]] | ||
| + | ====Characterization of Bba_K1314001, Bba_K1314013, Bba_K1314014 in Azotobacter vinelandii strain DJ==== | ||
| + | [[File:Picture2.png|800px]] | ||
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| − | <span class='h3bb'> | + | <span class='h3bb'></span> |
<partinfo>BBa_K1314001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1314001 SequenceAndFeatures</partinfo> | ||
Latest revision as of 04:04, 2 November 2014
nifH promoter with mRFP reporter
nifH promoter with RFP reporter.
Usage and Biology
Bba_K1314001 is comprised of nifH promoter and Bba_E1010. It is designed for testing nifH promoter. The right tube shows the expression of RFP using ptep promoter in Dh5a and the left tube show the expression of BbaK1314001 in DH5a. The result show that nifH promoter is not working in DH5a.
Characterization of Bba_K1314001, Bba_K1314013, Bba_K1314014 in Azotobacter vinelandii strain DJ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 801
Illegal AgeI site found at 913 - 1000COMPATIBLE WITH RFC[1000]