Difference between revisions of "Part:BBa K1332010"
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This part consists of the 5' side of the intron(+exon fragment) from td gene of T4 phage([https://parts.igem.org/Part:BBa_K1332004 BBa_K1332004]) and a double terminator([https://parts.igem.org/Part:BBa_B0015 BBa_B0015]). The protein coding sequence that is inserted between this device and mRNA circularization device (5’ side)([https://parts.igem.org/Part:BBa_K1332008 BBa_K1332008]) can be circularized. The 5' side of the intron(+exon fragment) from td gene of T4 phage contains a stop codon, so a circular mRNA made by this part <b>doesn’t cause continuous translation.</b> | This part consists of the 5' side of the intron(+exon fragment) from td gene of T4 phage([https://parts.igem.org/Part:BBa_K1332004 BBa_K1332004]) and a double terminator([https://parts.igem.org/Part:BBa_B0015 BBa_B0015]). The protein coding sequence that is inserted between this device and mRNA circularization device (5’ side)([https://parts.igem.org/Part:BBa_K1332008 BBa_K1332008]) can be circularized. The 5' side of the intron(+exon fragment) from td gene of T4 phage contains a stop codon, so a circular mRNA made by this part <b>doesn’t cause continuous translation.</b> | ||
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| + | <h3>explanation of this part</h3> | ||
| + | [[File:Gifu 5' not remove.png|500px|]]<br> | ||
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<h3>How to use</h3> | <h3>How to use</h3> | ||
Revision as of 03:53, 2 November 2014
mRNA circularization device (3 side)
This part consists of the 5' side of the intron(+exon fragment) from td gene of T4 phage(BBa_K1332004) and a double terminator(BBa_B0015). The protein coding sequence that is inserted between this device and mRNA circularization device (5’ side)(BBa_K1332008) can be circularized. The 5' side of the intron(+exon fragment) from td gene of T4 phage contains a stop codon, so a circular mRNA made by this part doesn’t cause continuous translation.
explanation of this part
How to use
Insert the protein coding sequence between this device and mRNA circularization device (5’ side)(BBa_K1332008).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]