Difference between revisions of "Part:BBa K1363603"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1363603 short</partinfo> | <partinfo>BBa_K1363603 short</partinfo> | ||
the gate is a ribozyme whose activity is repressed when combined with key-of-no | the gate is a ribozyme whose activity is repressed when combined with key-of-no | ||
− | + | <html> | |
+ | <style> | ||
+ | p { font-size: large; } | ||
+ | </style> | ||
+ | <p>The ribozyme is activated while the short RNA, key of no is no added. When lac is added, the short RNA combines with the ribozyme and restrains the cleavage, thus the level of GFP increases.</p> | ||
+ | <figure style="text-align:center"><img src="https://static.igem.org/mediawiki/2014/7/79/Ustc-2014-rna-NOTh.png" width="600" class="th" \><figcaption> | ||
+ | Fig.8 The principle of NO gate. | ||
+ | </figcaption></figure> | ||
+ | <figure style="text-align:center"><img src="https://static.igem.org/mediawiki/2014/6/61/Ustc-2014-rna-NO0.png" width="600" class="th" \><figcaption> | ||
+ | Fig.9 The schematic of NO gate. | ||
+ | </figcaption></figure> | ||
+ | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 03:36, 2 November 2014
gate of no of RNA logic gates
the gate is a ribozyme whose activity is repressed when combined with key-of-no
The ribozyme is activated while the short RNA, key of no is no added. When lac is added, the short RNA combines with the ribozyme and restrains the cleavage, thus the level of GFP increases.
![](https://static.igem.org/mediawiki/2014/7/79/Ustc-2014-rna-NOTh.png)
![](https://static.igem.org/mediawiki/2014/6/61/Ustc-2014-rna-NO0.png)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 129
Illegal SpeI site found at 154
Illegal PstI site found at 168 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 154
Illegal PstI site found at 168
Illegal NotI site found at 161 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 154
Illegal XbaI site found at 129 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 129
Illegal SpeI site found at 154
Illegal PstI site found at 168 - 1000COMPATIBLE WITH RFC[1000]