Difference between revisions of "Part:BBa K1555000"

Revision as of 01:00, 2 November 2014

The copa promoter wth a gfp reporter

This part is our testing part.The copa is one of the candidates of our copper sensor.For comparing with other two sequences,marO（BBa_K1555001） and cueo（BBa_K1555002）,we linked it with a reporter(BBa_E0840).With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measuring fluorescence intensity. 2h 0mg copper ion 2h 1mg copper ion

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 914

@@ Line 3: / Line 3: @@
 This part is our testing part.The ''copa'' is one of the candidates of our copper sensor.For comparing with other two sequences,''marO''（BBa_K1555001） and ''cueo''（BBa_K1555002）,we linked it with a reporter(BBa_E0840).With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measuring fluorescence intensity.
-[[File:2h 0mg.jpg]]   [[File:2h 1mg.jpg]]
+[[File:2h 0mg.jpg]] 2h 0mg copper ion   [[File:2h 1mg.jpg]] 2h 1mg copper ion
 <!-- Add more about the biology of this part here
 ===Usage and Biology===