Difference between revisions of "Part:BBa K1391009:Experience"

Latest revision as of 23:26, 1 November 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1391009

This part consists of Lyn fused via a glycine serine linker to a Tobacco Etch Virus (TEV) protease. It is designed for use in a B-Cell Receptor (BCR) tango system in which recruitment of Lyn will bring the TEV protease in close proximity to a TEV cleavage site fused to CD79A or CD79B and a transcription factor where it then cleaves the site.

User Reviews

UNIQ5b3eac9593ead568-partinfo-00000000-QINU UNIQ5b3eac9593ead568-partinfo-00000001-QINU

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 ===Applications of BBa_K1391009===
-This is fusion protein comprising Lyn and tobacco etch virus (TEV) protease, joined by a glycine-serine linker.
+This part consists of Lyn fused via a glycine serine linker to a Tobacco Etch Virus (TEV) protease. It is designed for use in a B-Cell Receptor (BCR) tango system in which recruitment of Lyn will bring the TEV protease in close proximity to a TEV cleavage site fused to CD79A or CD79B and a transcription factor where it then cleaves the site.
-B-cell receptors (BCRs) are naturally occurring, transmembrane protein complexes that consist of a membrane-bound antibody (IgM) and some associated proteins (CD79A and CD79B). Given that the variable region of the antibody can be specific for any of a large number of antigens, we designed a B-cell receptor to bind beta-amyloid plaques (a biomolecular hallmark of Alzheimer's disease). Once bound, activated receptors instigate intracellular signalling, which can then be manipulated to diagnose the disease.
 ===User Reviews===