Difference between revisions of "Part:BBa K1391109:Experience"

Latest revision as of 23:25, 1 November 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1391109

This is a fusion protein under a constitutive hEF1a promoter. It expresses Lyn fused via a glycine serine linker to a Tobacco Etch Virus (TEV) protease. It is designed for use in a B-Cell Receptor (BCR) tango system in which recruitment of Lyn will bring the TEV protease in close proximity to a TEV cleavage site fused to CD79A or CD79B and a transcription factor where it then cleaves the site.

User Reviews

UNIQdd299643929d07ac-partinfo-00000000-QINU UNIQdd299643929d07ac-partinfo-00000001-QINU

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 ===Applications of BBa_K1391109===
-This is a fusion protein under a constitutive hEF1a promoter. It expresses spleen tyrosine kinase (SYK) fused via a glycine serine linker to a Tobacco Etch Virus (TEV) protease. It is designed for use in a B-Cell Receptor (BCR) tango system in which recruitment of syk will bring the TEV protease in close proximity to a TEV cleavage site fused to CD79A or CD79B and a transcription factor where it then cleaves the site.
+This is a fusion protein under a constitutive hEF1a promoter. It expresses Lyn fused via a glycine serine linker to a Tobacco Etch Virus (TEV) protease. It is designed for use in a B-Cell Receptor (BCR) tango system in which recruitment of Lyn will bring the TEV protease in close proximity to a TEV cleavage site fused to CD79A or CD79B and a transcription factor where it then cleaves the site.
 ===User Reviews===