Difference between revisions of "Part:BBa K1391109:Experience"

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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===Applications of BBa_K1391109===
 
===Applications of BBa_K1391109===
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This part is under an inducible TRE promoter that requires the presence of both the activator rtTA and the antibiotic Doxycycline for transcription to occur.
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This is a fusion protein under a constitutive hEF1a promoter. It expresses Lyn fused via a glycine serine linker to a Tobacco Etch Virus (TEV) protease. It is designed for use in a B-Cell Receptor (BCR) tango system in which recruitment of Lyn will bring the TEV protease in close proximity to a TEV cleavage site fused to CD79A or CD79B and a transcription factor where it then cleaves the site.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 23:25, 1 November 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1391109

This is a fusion protein under a constitutive hEF1a promoter. It expresses Lyn fused via a glycine serine linker to a Tobacco Etch Virus (TEV) protease. It is designed for use in a B-Cell Receptor (BCR) tango system in which recruitment of Lyn will bring the TEV protease in close proximity to a TEV cleavage site fused to CD79A or CD79B and a transcription factor where it then cleaves the site.

User Reviews

UNIQ05a71f40883845b7-partinfo-00000000-QINU UNIQ05a71f40883845b7-partinfo-00000001-QINU