Difference between revisions of "Part:BBa K1391109:Experience"
(→‎Applications of BBa_K1391109)
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===Applications of BBa_K1391109===
 
===Applications of BBa_K1391109===
  
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This is fusion protein comprising Lyn and tobacco etch virus (TEV) protease, joined by a glycine-serine linker.
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This is a fusion protein under a constitutive hEF1a promoter. It expresses spleen tyrosine kinase (SYK) fused via a glycine serine linker to a Tobacco Etch Virus (TEV) protease. It is designed for use in a B-Cell Receptor (BCR) tango system in which recruitment of syk will bring the TEV protease in close proximity to a TEV cleavage site fused to CD79A or CD79B and a transcription factor where it then cleaves the site.
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This part is under an inducible TRE promoter that requires the presence of both the activator rtTA and the antibiotic Doxycycline for transcription to occur.
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B-cell receptors (BCRs) are naturally occurring, transmembrane protein complexes that consist of a membrane-bound antibody (IgM) and some associated proteins (CD79A and CD79B). Given that the variable region of the antibody can be specific for any of a large number of antigens, we designed a B-cell receptor to bind beta-amyloid plaques (a biomolecular hallmark of Alzheimer's disease). Once bound, activated receptors instigate intracellular signalling, which can then be manipulated to diagnose the disease.
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===User Reviews===
 
===User Reviews===

Revision as of 23:03, 1 November 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1391109

This is a fusion protein under a constitutive hEF1a promoter. It expresses spleen tyrosine kinase (SYK) fused via a glycine serine linker to a Tobacco Etch Virus (TEV) protease. It is designed for use in a B-Cell Receptor (BCR) tango system in which recruitment of syk will bring the TEV protease in close proximity to a TEV cleavage site fused to CD79A or CD79B and a transcription factor where it then cleaves the site.

User Reviews

UNIQ7bdf8d7bedad7848-partinfo-00000000-QINU UNIQ7bdf8d7bedad7848-partinfo-00000001-QINU