Difference between revisions of "Part:BBa K1539001:Experience"

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===Applications of BBa_K1539001===
 
===Applications of BBa_K1539001===
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BBa_K1539001 was created using devices XXX and YYY to insert relatively high expressing promoters and high efficiency RBS sites in front of mCherry from BBa_J0650.  Expression of mCherry was quantified by flow cytometry using a FACS AriaIII.  Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry and the median intensity was 4X brighter than a low promoter/weak RBS combination (see part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1539002]).  Our raw data is summarized below:
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BBa_K1539001 was created using devices BBa_K1539021 and BBa_K1539055 to insert a relatively high expressing promoter and a high efficiency RBS site in front of mCherry from BBa_J0650.  Expression of mCherry was quantified by flow cytometry using a FACS AriaIII.  Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, YYY% of our cells expressed mCherry and the median intensity was 4X brighter than a low promoter/weak RBS combination (see part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1539002]).  Our raw data is summarized below:
  
 
"High Expression Combination" - Primers PH1/RH1:
 
"High Expression Combination" - Primers PH1/RH1:
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[[File:file:///C:/Users/yannis/Downloads/140821_DMC_mCherry_PH1-RH1.pdf]]
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Negative Control:
 
Negative Control:
  

Revision as of 21:02, 1 November 2014

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Please enter how you used this part and how it worked out.

Applications of BBa_K1539001

BBa_K1539001 was created using devices BBa_K1539021 and BBa_K1539055 to insert a relatively high expressing promoter and a high efficiency RBS site in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, YYY% of our cells expressed mCherry and the median intensity was 4X brighter than a low promoter/weak RBS combination (see part [1]). Our raw data is summarized below:

"High Expression Combination" - Primers PH1/RH1:


Negative Control:

User Reviews

UNIQf9baf1ff07fb7953-partinfo-00000000-QINU UNIQf9baf1ff07fb7953-partinfo-00000001-QINU