Difference between revisions of "Part:BBa K1539002:Experience"

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===Applications of BBa_K1539002===
 
===Applications of BBa_K1539002===
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BBa_K1539001 was created using devices BBa_K1539034 and BBa_K1539089 to insert relatively high expressing promoters and high efficiency RBS sites in front of mCherry from BBa_J0650.  Expression of mCherry was quantified by flow cytometry using a FACS AriaIII.  Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry and the median intensity was 4X dimmer than a high promoter/high RBS combination (see part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1539001]).  Our raw data is summarized below:
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BBa_K1539001 was created using devices BBa_K1539034 and BBa_K1539089 to insert relatively a low expressing promoter and low efficiency RBS site in front of mCherry from BBa_J0650.  Expression of mCherry was quantified by flow cytometry using a FACS AriaIII.  Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry, and the median intensity was 4X dimmer than a high promoter/high RBS combination (see part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1539001]).  Our raw data is summarized below:
  
 
"Low Expression Combination" - Primers PL1/RL1:
 
"Low Expression Combination" - Primers PL1/RL1:
  
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[[140821_DMC_mCherry_PL1-RL1.pdf]]
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[[File:140821_DMC_mCherry_PL1-RL1.pdf]]
  
 
Negative Control:
 
Negative Control:
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[[File:140821_DMC_mCherry_Promoterless.pdf]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 21:00, 1 November 2014


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1539002

BBa_K1539001 was created using devices BBa_K1539034 and BBa_K1539089 to insert relatively a low expressing promoter and low efficiency RBS site in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry, and the median intensity was 4X dimmer than a high promoter/high RBS combination (see part [1]). Our raw data is summarized below:

"Low Expression Combination" - Primers PL1/RL1:

File:140821 DMC mCherry PL1-RL1.pdf

Negative Control:

File:140821 DMC mCherry Promoterless.pdf

User Reviews

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