Difference between revisions of "Part:BBa K1539002:Experience"
Stassoulas3 (Talk | contribs) (→Applications of BBa_K1539002) |
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"Low Expression Combination" - Primers PL1/RL1: | "Low Expression Combination" - Primers PL1/RL1: | ||
| − | [[140821_DMC_mCherry_PL1-RL1.pdf]] | + | [[File:140821_DMC_mCherry_PL1-RL1.pdf]] |
Negative Control: | Negative Control: | ||
Revision as of 20:36, 1 November 2014
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1539002
BBa_K1539001 was created using devices BBa_K1539034 and BBa_K1539089 to insert relatively high expressing promoters and high efficiency RBS sites in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry and the median intensity was 4X dimmer than a high promoter/high RBS combination (see part [1]). Our raw data is summarized below:
"Low Expression Combination" - Primers PL1/RL1:
File:140821 DMC mCherry PL1-RL1.pdf
Negative Control:
User Reviews
UNIQ1a6f058b8e38d2c3-partinfo-00000000-QINU UNIQ1a6f058b8e38d2c3-partinfo-00000001-QINU