Difference between revisions of "Part:BBa K1539002:Experience"
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===Applications of BBa_K1539002=== | ===Applications of BBa_K1539002=== | ||
| − | BBa_K1539001 was created using devices XXX and YYY to insert relatively high expressing promoters and high efficiency RBS sites in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry and the median intensity was 4X | + | BBa_K1539001 was created using devices XXX and YYY to insert relatively high expressing promoters and high efficiency RBS sites in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry and the median intensity was 4X dimmer than a high promoter/high RBS combination (see part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1539001]). Our raw data is summarized below: |
"Low Expression Combination" - Primers PL1/RL1: | "Low Expression Combination" - Primers PL1/RL1: | ||
| − | file:///C:/Users/yannis/Downloads/140821_DMC_mCherry_PL1-RL1.pdf | + | [[File:file:///C:/Users/yannis/Downloads/140821_DMC_mCherry_PL1-RL1.pdf]] |
Negative Control: | Negative Control: | ||
Revision as of 20:32, 1 November 2014
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Applications of BBa_K1539002
BBa_K1539001 was created using devices XXX and YYY to insert relatively high expressing promoters and high efficiency RBS sites in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry and the median intensity was 4X dimmer than a high promoter/high RBS combination (see part [1]). Our raw data is summarized below:
"Low Expression Combination" - Primers PL1/RL1:
File:File:///C:/Users/yannis/Downloads/140821 DMC mCherry PL1-RL1.pdf
Negative Control:
User Reviews
UNIQd1d3169b7f214678-partinfo-00000000-QINU UNIQd1d3169b7f214678-partinfo-00000001-QINU