Difference between revisions of "Part:BBa J45600:Design"
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Latest revision as of 05:56, 31 October 2006
Stationary phase banana odor biosynthetic system
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3204
Illegal EcoRI site found at 4105
Illegal EcoRI site found at 4804
Illegal EcoRI site found at 4990
Illegal SpeI site found at 3898 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3204
Illegal EcoRI site found at 4105
Illegal EcoRI site found at 4804
Illegal EcoRI site found at 4990
Illegal SpeI site found at 3898 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3204
Illegal EcoRI site found at 4105
Illegal EcoRI site found at 4804
Illegal EcoRI site found at 4990
Illegal BglII site found at 327
Illegal BamHI site found at 1654 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3204
Illegal EcoRI site found at 4105
Illegal EcoRI site found at 4804
Illegal EcoRI site found at 4990
Illegal SpeI site found at 3898 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3204
Illegal EcoRI site found at 4105
Illegal EcoRI site found at 4804
Illegal EcoRI site found at 4990
Illegal SpeI site found at 3898
Illegal AgeI site found at 3068 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 754
Illegal SapI.rc site found at 1762
Design Notes
We recommend use of strain BBa_J45999 as it does not produce indole. Indole is responsible for the foul smell of wt e.coli, and masks the banana scent produced by J45600.
Source
This system is composed of devices J45250 and J45400.
References
ATF1
<biblio> ATF1
- Horton03 pmid=12937998
BAT2
- BAT2 pmid=12172617
THI3
- THI3 pmid=12172617
</biblio>