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Software error:

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Status: 500 Content-type: text/html

Software error:

Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 85.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

Design Notes

Primers used to PCR this part out of the E.coli genome:

Forward Primer

• 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3'
• Total Length: 47 bp
• Annealing Length: 19 bp
• GC Content: 52.6%
• Melting Temperature: 53.4 degrees C
• hairpin deltaG: -3.99 kcal/mol
• self dimer deltaG: -98.3 kcal/mol

Reverse Primer

• 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3'
• Total Length: 54 bp
• Annealing Length: 25 bp
• GC Content: 20.0%
• Melting Temperature: 46.4 degrees C
• hairpin deltaG: -2.86 kcal/mol
• self dimer deltaG: -99.44 kcal/mol
• heterodimer deltaG with Forward Primer: -99.44 kcal/mol

Source

This part was PCRed out of E. coli's genome with the primers listed in the design notes section.

References

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Nucleotide&list_uids=453110&dopt=GenBank

http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=8282684

http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=9829938

http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2958.2002.02746.x

http://www.pubmedcentral.org/articlerender.fcgi?artid=178012

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3C-456T3CY-3&_coverDate=04%2F25%2F2002&_alid=423325879&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=4943&_sort=d&view=c&_acct=C000022659&_version=1&_urlVersion=0&_userid=501045&md5=7d6f4bac2242f1baa6b2b15260904531

Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.