Difference between revisions of "Part:BBa J45200"
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Protein generator producing the protein ATF1 under control of the LacI repressor.  Thus, in a LacI<sup>-</sup> ''E. coli'' strain, ATF1 is continually produced.  ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols (including isoamyl acetate from isoamyl alcohol).  This part is almost identical to <partinfo>J45220</partinfo>  except that it uses the B0030 RBS (instead of B0032 as is in <partinfo>J45220</partinfo>).  The Banana Generating Device (BGD) function thus far has been to convert isoamyl alcohol into isoamyl acetate (a banana-scented compound).
 
Protein generator producing the protein ATF1 under control of the LacI repressor.  Thus, in a LacI<sup>-</sup> ''E. coli'' strain, ATF1 is continually produced.  ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols (including isoamyl acetate from isoamyl alcohol).  This part is almost identical to <partinfo>J45220</partinfo>  except that it uses the B0030 RBS (instead of B0032 as is in <partinfo>J45220</partinfo>).  The Banana Generating Device (BGD) function thus far has been to convert isoamyl alcohol into isoamyl acetate (a banana-scented compound).
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[[Image:J45200.jpg]]
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<!-- Add more about the biology of this part here
 
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Revision as of 05:25, 31 October 2006

Banana odor generator

Protein generator producing the protein ATF1 under control of the LacI repressor. Thus, in a LacI- E. coli strain, ATF1 is continually produced. ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols (including isoamyl acetate from isoamyl alcohol). This part is almost identical to BBa_J45220 except that it uses the B0030 RBS (instead of B0032 as is in BBa_J45220). The Banana Generating Device (BGD) function thus far has been to convert isoamyl alcohol into isoamyl acetate (a banana-scented compound).

J45200.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 182
    Illegal BamHI site found at 1509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 609
    Illegal SapI.rc site found at 1617