Difference between revisions of "Part:BBa K1351034"

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<partinfo>BBa_K1351034 short</partinfo>
 
<partinfo>BBa_K1351034 short</partinfo>
  
"This part was generated in a modified version of RFC10, and has the following prefix and suffix:
 
  
{|
+
This part was generated in RFC10, and has the following prefix and suffix:  
|prefix with EcoRI, NotI, XbaI and NgoMIV:
+
|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>TG<span style="color:orange">GCCGGC</span>
+
              Prefix                                                                  Suffix
 +
5' - GAATTC GCGGCCGC T TCTAGA G aagaagagata T ACTAGT A GCGGCCG CTGCAG - 3'
 +
      EcoRI    NotI      XbaI                                        SpeI    NotI  PstI
 +
 
 +
 
 +
This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
 +
This library was generated utilizing the [https://salis.psu.edu/software/ Salis lab RBS calculator] to find RBS that cover a high space of translation intitiation rate in 'B. subtilis'. It includes seven RBS with the following sequences:
 +
 
 +
{| class=wikitable
 
|-
 
|-
|suffix with AgeI, SpeI, NotI and PstI:
+
!Number
|<span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">AC<u>TAG</u><u>T</u></span><u>A</u><span style="color:green"><u>G</u>CGGCCG</span><span style="color:blue">CTGCAG</span>
+
!BBa_K1310 ..
 +
!Sequence
 +
|-
 +
|1
 +
|28
 +
|aaggagggata
 +
|-
 +
|2
 +
|29
 +
|agaggaggata
 +
|-
 +
|3
 +
|30
 +
|aaggagagata
 +
|-
 +
|4
 +
|31
 +
|aggagaggata
 +
|-
 +
|5
 +
|32
 +
|aagaggagata
 +
|-
 +
|6
 +
|33
 +
|agaaagggata
 +
|-
 +
|<b>7</b>
 +
|<b>34</b>
 +
|<b>aagaagagata</b>
 
|}
 
|}
Sites of restriction enzymes generating compatible overhangs have the same color:
 
  
<span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red, <span style="color:orange">NgoMIV</span> and <span style="color:orange">AgeI</span> in orange. Stop codons are underlined.
+
The coverage of RBS strength as calculated by the [https://salis.psu.edu/software/ RBS calculator] is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.
 +
 
 +
[[File:LMU_14_RBSlibrary.png|thumb|center|400px|Fig.1: Coverage of the translation initiation rate of the RBS library as calculated by the [https://salis.psu.edu/software/ RBS calculator]]]
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 06:42, 1 November 2014

LMU Bacillus RBS collection 7


This part was generated in RFC10, and has the following prefix and suffix:

             Prefix                                                                   Suffix
5' - GAATTC GCGGCCGC T TCTAGA G aagaagagata T ACTAGT A GCGGCCG CTGCAG - 3'
     EcoRI    NotI      XbaI                                         SpeI     NotI   PstI 


This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus]. This library was generated utilizing the Salis lab RBS calculator to find RBS that cover a high space of translation intitiation rate in 'B. subtilis'. It includes seven RBS with the following sequences:

Number BBa_K1310 .. Sequence
1 28 aaggagggata
2 29 agaggaggata
3 30 aaggagagata
4 31 aggagaggata
5 32 aagaggagata
6 33 agaaagggata
7 34 aagaagagata

The coverage of RBS strength as calculated by the RBS calculator is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.

Fig.1: Coverage of the translation initiation rate of the RBS library as calculated by the RBS calculator


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]