Difference between revisions of "Part:BBa K1351031"

Latest revision as of 06:37, 1 November 2014

LMU Bacillus RBS collection 4

This part was generated in RFC10, and has the following prefix and suffix:

             Prefix                                                                   Suffix
5' - GAATTC GCGGCCGC T TCTAGA G aggagaggata T ACTAGT A GCGGCCG CTGCAG - 3'
     EcoRI    NotI      XbaI                                         SpeI     NotI   PstI

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

This library was generated utilizing the Salis lab RBS calculator to find RBS that cover a high space of translation intitiation rate in 'B. subtilis'. It includes seven RBS with the following sequences:

Number	BBa_K1310 ..	Sequence
1	28	aaggagggata
2	29	agaggaggata
3	30	aaggagagata
4	31	aggagaggata
5	32	aagaggagata
6	33	agaaagggata
7	34	aagaagagata

The coverage of RBS strength as calculated by the RBS calculator is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.

Fig.1: Coverage of the translation initiation rate of the RBS library as calculated by the RBS calculator

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1351031 short</partinfo>
-"This part was generated in a modified version of RFC25, and has the following prefix and suffix:
+This part was generated in RFC10, and has the following prefix and suffix:
+              Prefix                                                                   Suffix
+' - GAATTC GCGGCCGC T TCTAGA G aggagaggata T ACTAGT A GCGGCCG CTGCAG - 3'
+      EcoRI    NotI      XbaI                                         SpeI     NotI   PstI
-{|
+This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
-|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:
-|<span style=""color:blue"">GAATTC</span><span style=""color:green"">GCGGCCGC</span>T<span style=""color:red"">TCTAGA</span>TG<span style=""color:orange"">GCCGGC</span>
+This library was generated utilizing the [https://salis.psu.edu/software/ Salis lab RBS calculator] to find RBS that cover a high space of translation intitiation rate in 'B. subtilis'. It includes seven RBS with the following sequences:
+{| class=wikitable
 |-
-|suffix with AgeI, SpeI, NotI and PstI:
+!Number
-|<span style=""color:orange"">ACCGGT</span><u>TAA</u>T<span style=""color:red"">AC<u>TAG</u><u>T</u></span><u>A</u><span style=""color:green""><u>G</u>CGGCCG</span><span style=""color:blue"">CTGCAG</span>
+!BBa_K1310 ..
+!Sequence
+|-
+|1
+|28
+|aaggagggata
+|-
+|2
+|29
+|agaggaggata
+|-
+|3
+|30
+|aaggagagata
+|-
+|<b>4</b>
+|<b>31</b>
+|<b>aggagaggata</b>
+|-
+|5
+|32
+|aagaggagata
+|-
+|6
+|33
+|agaaagggata
+|-
+|7
+|34
+|aagaagagata
 |}
-Sites of restriction enzymes generating compatible overhangs have the same color:
-<span style=""color:blue"">EcoRI</span> and <span style=""color:blue"">PstI</span> in blue, <span style=""color:green"">NotI</span> in green, <span style=""color:red"">XbaI</span> and <span style=""color:red"">SpeI</span> in red, <span style=""color:orange"">NgoMIV</span> and <span style=""color:orange"">AgeI</span> in orange. Stop codons are underlined."
+The coverage of RBS strength as calculated by the [https://salis.psu.edu/software/ RBS calculator] is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.
+[[File:LMU_14_RBSlibrary.png|thumb|center|400px|Fig.1: Coverage of the translation initiation rate of the RBS library as calculated by the [https://salis.psu.edu/software/ RBS calculator]]]