Difference between revisions of "Part:BBa K1351038"
 
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This part belongs to the of the AIP-II sensing two component system [https://parts.igem.org/Part:BBa_K1351036 AgrCA]. Upon detection of autoinducing peptide II (AIP-II), AgrC phosphorylates AgrA, which then bindes to distinct binding sites and activates the [https://parts.igem.org/Part:BBa_K1351037 P2] and P3 [this part] promoters.
 
This part belongs to the of the AIP-II sensing two component system [https://parts.igem.org/Part:BBa_K1351036 AgrCA]. Upon detection of autoinducing peptide II (AIP-II), AgrC phosphorylates AgrA, which then bindes to distinct binding sites and activates the [https://parts.igem.org/Part:BBa_K1351037 P2] and P3 [this part] promoters.
  
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This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
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[[File:LMU14 Cap2p3.png|600px]]
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Luminescence of ''B. subtilis'' P<sub>''xylA''</sub> - [https://parts.igem.org/Part:BBa_K1351037 ''agrCA''] / [https://parts.igem.org/Part:BBa_K1351037 P2]-''lux'' and [https://parts.igem.org/Part:BBa_K1351038 P3]-''lux'' was measured in MCSE induced with 0, 0.02, 0.2 % xylose. This plot shows the luminescence readout normalised over OD600 over the course of 6hours. The higher output in xylose induced samples shows the successfull expression of ''agrCA'' in ''B. subtilis''.
  
 
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Latest revision as of 04:55, 1 November 2014

P3-promoter

Quorum sensing dependant promotor (AIP II). Regulated by phosphorylated AgrA. Contains four AgrA binding sites upstream. This part belongs to the of the AIP-II sensing two component system AgrCA. Upon detection of autoinducing peptide II (AIP-II), AgrC phosphorylates AgrA, which then bindes to distinct binding sites and activates the P2 and P3 [this part] promoters.

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

LMU14 Cap2p3.png

Luminescence of B. subtilis PxylA - agrCA / P2-lux and P3-lux was measured in MCSE induced with 0, 0.02, 0.2 % xylose. This plot shows the luminescence readout normalised over OD600 over the course of 6hours. The higher output in xylose induced samples shows the successfull expression of agrCA in B. subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]